Principal investigator
Name:
Guanjun GaoAssociate Professor , PhD, Associate Professor
Position:
Affiliation:
School of Life Science and Technology
Honor:
Group Introduction
Research Area:
Chromatin and Epigenetics, Gene Editing
Research Interests:
The major mission of Gao-lab is to use Drosophila and mammalian cell systems to achieve a comprehensive understanding of the mechanisms of chromatin and (epi-)genetic factors, particularly with a focus on long non-coding RNAs and histone epigenetic codes in development, reproduction, innate immunology, aging and cancer disease. Towards achieving this mission, Gao-lab bridges the new genetic methods and biochemistry approaches into investigation of the mechanisms of those (epi-)genetics factors involved in fundamental biological processes. Their research will define not only epigenetic molecules and pathways but also many fundamental biological processes that impact human health.
Representative Publications (*First Author, # Corresponding Author)
- 1. Angyang Sun；Zilong Wei；Jie Zheng；Xuedi Zhang#；Guanjun Gao#.A histone H2A and H2B point mutant library defines essential in vivo functions in Drosophila.Cell Reports. 23 December 2025.
- 2. Xuedi Zhang*, Hongyun Liu*, Ju Peng*, Guangbin Wang*, Xiangyu Wu**, Shuai Li, Jie Zheng, Qianwen Sun ,Guanjun Gao#.Adar contributes to genome integrity by regulating R-loop homeostasis in Drosophila.BMC Biology. 15 July 2025.
- 3. Ju Peng** ; Angyang Sun ; Jie Zheng ; Na Zhang, ; Xuedi Zhang# ; Guanjun Gao##.Testis-specific serine/threonine kinase dTSSK2 regulates sperm motility and male fertility in Drosophila.Communications Biology. 08 May 2025.
- 4. Wangfei Shi* ; Xuedi Zhang*# ; Angyang Sun* ; Jie Zheng ; Kailong Zhuang ; Ziheng Chen ; Ju Peng ; Pengchong Fu ; Guanjun Gao##.A powerful and highly efficient PAI-mediated transgenesis approach in Drosophila.Nucleic Acids Research. 23 April 2025.
- 5. Mingyao Wang* , Pengchong Fu*, Ziheng Chen* , Xiangnan Wang*, Hanhui Ma , Xuedi Zhang# , Guanjun Gao# .Recruitment and rejoining of remote double-strand DNA breaks for enhanced and precise chromosome editing.Genome Biology. 2025 Mar.
- 6. Xuedi Zhang, Ju Peng, Menghua Wu, Angyang Sun, Xiangyu Wu, Jie Zheng, Wangfei Shi & Guanjun Gao.Broad phosphorylation mediated by testis-specific serine/threonine kinases contributes to spermiogenesis and male fertility.Nature Communications. 06 May 2023.
- 7. Xuedi Zhang, Xiangyu Wu, Ju Peng, Angyang Sun, Yan Guo, Pengchong Fu and Guanjun Gao.Cis- and trans-regulation by histone H4 basic patch R17/R19 in metazoan development.Open Biology. 12 Nov 2022 .
- 8. Gao, Kaixuan; Zhang, Xuedi; Zhang, Zhenwu; Wu, Xiangyu; Guo, Yan; Fu, Pengchong; Sun, Angyang; Peng, Ju; Zheng, Jie; Yu, Pengfei; Wang, Tengfei; Ye, Qinying; Jiang, Jingwei; Wang, Haopeng; Lin, Chao-Po; Gao, Guanjun##.Transcription-coupled donor DNA expression increases homologous recombination for efficient genome editing.Nucleic Acids Research. 01 Aug 2022.
- 9. Yan, Cheng*; Liu, Jingqi; Gao, Jiamei; Sun, Ying; Zhang, Lei; Song, Haiyun; Xue, Lei; Zhan, Lixing; Gao, Guanjun; Ke, Zunji; Liu, Yong; Liu, Jingnan.IRE1 promotes neurodegeneration through autophagy-dependent neuron death in the Drosophila model of Parkinson's disease.CELL DEATH & DISEASE. 22 Oct 2019. 10(11).
- 10. Zhang, Weimin*; Zhang, Xuedi*; Xue, Zhaoyu*; Li, Yijie*; Ma, Qing; Ren, Xiangle; Zhang, Jiaying; Yang, Songhua; Yang, Lijuan; Wu, Menghua; Ren, Mengda; Xi, Rongwen; Wu, Zheng; Liu, Ji-Long; Matunis, Erika; Dai, Junbiao; Gao, Guanjun.Probing the Function of Metazoan Histones with a Systematic Library of H3 and H4 Mutants.DEVELOPMENTAL CELL. 11 Feb 2019. 48(3):406-419.
- 11. Wu, Menghua*; Zhang, Xuedi; Wei, Wei; Long, Li; An, Sainan; Gao, Guanjun.CRISPR/Cas9 mediated genetic resource for unknown kinase and phosphatase genes in Drosophila.SCIENTIFIC REPORTS. 30 Apr 2020. 10(1).
- 12. Bhaskar Roy, Jing Zhao, Chao Yang , Wen Luo , Teng Xiong , Yong Li , Xiaodong Fang , Guanjun Gao, Chabungbam O. Singh , Lise Madsen , Yong Zhou and Karsten Kristiansen.CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities.Frontiers in Genetics. 05 July 2018.
- 13. Xiao, Xiaoping*; Yang, Lijuan*; Pang, Xiaojing*; Zhang, Rudian; Zhu, Yibin; Wang, Penghua; Gao, Guanjun; Cheng, Gong.A Mesh-Duox pathway regulates homeostasis in the insect gut.NATURE MICROBIOLOGY. 2017. 2(5).
- 14. Lianxin Hu, Jiajun Xu, Meng-Xin Yin, Liguo Zhang, Yi Lu, Wenqing Wu, Zhaoyu Xue, Margaret S Ho, Guanjun Gao, Yun Zhao & Lei Zhang.Ack promotes tissue growth via phosphorylation and suppression of the Hippo pathway component Expanded.Cell Discovery. 23 February 2016.
- 15. Ruijun Tang ；Mengqi Zhou ； Yuwei Chen ；Zhenghui Jiang ； Xunan Fan ； Jingheng Zhang ； Aiping Dong ；Lu Lv；Song Mao；Fang Chen；Guanjun Gao ；Jinrong Min ； Ke Liu；Kai Yuan.H3K14ac facilitates the reinstallation of constitutive heterochromatin in Drosophila early embryos by engaging Eggless/SetDB1.Proc Natl Acad Sci U S A. 2024 Aug 6.
- 16. Wen, Kejia*; Yang, Lijuan*; Xiong, Tuanlin*; Di, Chao; Ma, Danhui; Wu, Menghua; Xue, Zhaoyu; Zhang, Xuedi; Long, Li; Zhang, Weimin; Zhang, Jiaying; Bi, Xiaolin; Dai, Junbiao; Zhang, Qiangfeng; Lu, Zhi John; Gao, Guanjun.Critical roles of long noncoding RNAs in Drosophila spermatogenesis.GENOME RESEARCH. 2016. 26(9):1233-1244.
- 17. Xue, Zhaoyu*; Wu, Menghua; Wen, Kejia; Ren, Menda; Long, Li; Zhang, Xuedi; Gao, Guanjun.CRISPR/Cas9 Mediates Efficient Conditional Mutagenesis in Drosophila.G3-GENES GENOMES GENETICS. 2014. 4(11):2167-2173.
- 18. Gerstein, Mark B.*; Rozowsky, Joel; Yan, Koon-Kiu; Wang, Daifeng; Cheng, Chao; Brown, James B.; Davis, Carrie A.; Hillier, LaDeana; Sisu, Cristina; Li, Jingyi Jessica; Pei, Baikang; Harmanci, Arif O.; Duff, Michael O.; Djebali, Sarah; Alexander, Roger P.; Alver, Burak H.; Auerbach, Raymond; Bell, Kimberly; Bickel, Peter J.; Boeck, Max E.; Boley, Nathan P.; Booth, Benjamin W.; Cherbas, Lucy; Cherbas, Peter; Di, Chao; Dobins, Alex; Drenkows, Jorg; Ewing, Brent; Fang, Gang; Fastucas, Megan; Feingold, Elise A.; Frankish, Adam; Gao, Guanjun; Good, Peter J.; Guigo, Roderic; Hammonds, Ann; Harrow, Jen; Hoskins, Roger A.; Howald, Cedric; Hu, Long; Huang, Haiyan; Hubbard, Tim J. P.; Huynh, Chau; Jhas, Sonali; Kasper, Dionna; Kato, Masaomi; Kaufman, Thomas C.; Kitchen, Robert R.; Ladewig, Erik; Lagarde, Julien; Lai, Eric; Leng, Ling; Lu, Zhi; MacCoss, Michael; May, Gemma; McWhirter, Rebecca; Merrihew, Gennifer; Miller, David M.; Mortazavi, Ali; Murad, Rabi; Oliver, Brian; Olson, Sara; Park, Peter J.; Pazin, Michael J.; Perrimon, Norbert; Pervouchine, Dmitri; Reinke, Valerie; Reymond, Alexandre; Robinson, Garrett; Samsonova, Anastasia; Saunders, Gary I.; Schlesingers, Felix; Sethi, Anurag; Slack, Frank J.; Spencer, William C.; Stoiber, Marcus H.; Strasbourger, Pnina; Tanzer, Andrea; Thompson, Owen A.; Wan, Kenneth H.; Wang, Guilin; Wang, Huaien; Watkins, Kathie L.; Wen, Jiayu; Wen, Kejia; Xue, Chenghai; Yang, Li; Yip, Kevin; Zaleskis, Chris; Zhang, Yan; Zheng, Henry; Brenner, Steven E.; Graveley, Brenton R.; Ceniker, Susan E.; Gingeras, Thomas R.; Waterston, Robert.Comparative analysis of the transcriptome across distant species.NATURE. 2014. 512(7515):445-448.
- 19. Xue, Zhaoyu*; Ren, Mengda; Wu, Menghua; Dai, Junbiao; Rong, Yikang S.; Gao, Guanjun.Efficient Gene Knock-out and Knock-in with Transgenic Cas9 in Drosophila.G3-GENES GENOMES GENETICS. 2014. 4(5):925-929.
- 20. Wei, Wei*; Xin, Huhu; Roy, Bhaskar; Dai, Junbiao; Miao, Yungen; Gao, Guanjun.Heritable Genome Editing with CRISPR/Cas9 in the Silkworm, Bombyx mori.PLOS ONE. 2014. 9(7).
- 21. Yu, Zhongsheng*; Ren, Mengda*; Wang, Zhanxiang; Zhang, Bo; Rong, Yikang S.; Jiao, Renjie; Gao, Guanjun.Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila.GENETICS. 2013. 195(1):2890.
- 22. Wei, Chuanxian*; Liu, Jiyong; Yu, Zhongsheng; Zhang, Bo; Gao, Guanjun; Jiao, Renjie.TALEN or Cas9-Rapid, Efficient and Specific Choices for Genome Modifications.JOURNAL OF GENETICS AND GENOMICS. 2013. 40(6):281-289.
- 23. Ke Shui, Chenwei Wang, Xuedi Zhang, Shanshan Ma, Qinyu Li, Wanshan Ning, Weizhi Zhang, Miaomiao Chen, Di Peng, Hui Hu, Zheng Fang, Anyuan Guo, Guanjun Gao, Mingliang Ye, Luoying Zhang & Yu Xue.Small-sample learning reveals propionylation in determining global protein homeostasis.Nature Communications. 17 May 2023.
- 24. Gao, Guanjun*; Cheng, Yan; Wesolowska, Natalia; Rong, Yikang S..Paternal imprint essential for the inheritance of telomere identity in Drosophila.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2011. 108(12):4932-4937.
- 25. Gao, Guanjun*; Walser, Jean-Claude; Beaucher, Michelle L.; Morciano, Patrizia; Wesolowska, Natalia; Chen, Jie; Rong, Yikang S..HipHop interacts with HOAP and HP1 to protect Drosophila telomeres in a sequence-independent manner.EMBO JOURNAL. 2010. 29(4):819-829.
- 26. Gao, Guanjun*; Bi, Xiaolin; Chen, Jie; Srikanta, Deepa; Rong, Yikang S..Mre11-Rad50-Nbs complex is required to cap telomeres during Drosophila embryogenesis.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2009. 106(26):10728-10733.
- 27. Gao, Guanjun*; McMahon, Conor; Chen, Jie; Rong, Yikang S..A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008. 105(37):13999-14004.
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